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. 2019 Dec 30;295(6):1539–1550. doi: 10.1074/jbc.RA119.011605

Figure 3.

Figure 3.

Knockdown of TgUroD reduces free heme and mitochondrial c-type cytochrome abundance in parasites. A, free heme levels were measured in parasites grown in the absence (blue) or presence (orange) of ATc for 3 days. Data show the mean ± S.D. from three independent experiments (*, p < 0.05; two-tailed unpaired Student's t test). B, Western blots of rTgUroD-c-myc/TgCyt c-A-HA parasites grown in the presence of ATc for 0–4 days and probed with antibodies against c-Myc, HA and TgGRA8 (loading control). C, Western blots of rTgUroD/TgCyt c-B-HA parasites grown in the presence of ATc for 0–4 days and probed with antibodies against HA and anti-TgTom40 (loading control). D, Western blots of rTgUroD/TgCyt c1-c-Myc parasites grown in the presence of ATc for 0–4 days and probed with antibodies against c-Myc and anti-TgTom40 (loading control). All Western blots are representative of three independent experiments.