Figure 8.

Hsp104 NBD2 cryo-EM models colored based on XF modification rates. A–C, hexameric NBD2 domains were modeled based on cryo-EM studies (16) and colored based on the XF modification rates with blue as unmodified, yellow less than 1 s⁻¹, dark yellow 1–10 s⁻¹, orange 10–50 s⁻¹, red more than 50 s⁻¹, and gray is no coverage for the hexamer with ADP (A, PDB code 5vy8) and ATPγS in the closed (B, PDB code 5vjh) and extended conformations (C, PDB code 5vya).