Table 2.
Selected peptide list
Peptides that are found in two or more states, which cover important regions of Hsp104, are shown. Normalized modification rates and modified residues are listed side by side for ease of comparison. ND means not determined and pnf means peptide not found.
| Start | End | Monomer |
Hexamer with ADP |
Hexamer with ATPγS |
|||
|---|---|---|---|---|---|---|---|
| Modified residues (identified by MS2) | Modification rate (s−1) | Modified residues (identified by MS2) | Modification rate (s−1) | Modified residues (identified by MS2) | Modification rate (s−1) | ||
| 8 | 38 | 0.03 ± 0.04 | 0 | 1.85 ± 0.33 | |||
| 39 | 53 | pnf | 25.02 ± 0.01 | 4.28 ± 1.50 | |||
| 55 | 64 | 0 | 3.60 ± 0.01 | 0.86 ± 0.18 | |||
| 86 | 110 | 87, 88, 90, 91, 95, 96, 100, 101, 104, 108 | 0.80 ± 0.35 | 90, 94, 96, 102, 107 | 0.59 ± 0.01 | 87, 90, 91, 94, 96, 103, 109 | 5.35 ± 0.35 |
| 121 | 134 | 0 | 6.48 ± 0.29 | 1.15 ± 0.41 | |||
| 148 | 166 | 0.22 ± 0.01 | 0.10 ± 0.002 | 0 | |||
| 211 | 223 | 0 | 32.61 ± 1.89 | 0 | |||
| 225 | 243 | 15.90 ± 11.23 | 0 | 0 | |||
| 282 | 299 | ND | 285, 286, 288, 289, 290, 292, 294, 298, 299 | 74.97 ± 0.59 | ND | ||
| 283 | 299 | ND | 28.07 ± 0.42 | 0 | |||
| 286 | 310 | ND | 26.91 ± 0.33 | 0 | |||
| 300 | 320 | ND | 145.58 ± 3.38 | 0 | |||
| 311 | 320 | ND | 311 | 2.28 ± 0.03 | 311 | 0.37 ± 0.07 | |
| 331 | 350 | 0 | 133.54 ± 3.37 | 0 | |||
| 332 | 349 | 2.37 ± 0.29 | 333 | 3.77 ± 0.26 | 0 | ||
| 405 | 414 | pnf | 21.51 ± 0.39 | 52.81 ± 9.52 | |||
| 415 | 423 | pnf | 415, 416, 417, 418, 421, 423 | 35.51 ± 0.79 | 0 | ||
| 431 | 455 | 0.22 ± 0.10 | 0.57 ± 0.01 | 0.08 ± 0.05 | |||
| 467 | 493 | 0 | 0 | 0.84 ± 0.12 | |||
| 505 | 526 | pnf | 349.14 ± 0.95 | 0 | |||
| 506 | 522 | 6.20 ± 4.30 | 20.14 ± 1.23 | 1.51 ± 1.04 | |||
| 508 | 536 | ND | 511, 517, 523 | 88.81 ± 3.41 | ND | ||
| 611 | 631 | 613, 615, 626 | 2.30 ± 0.76 | 4.94 ± 2.23 | 0 | ||
| 613 | 623 | 0 | 0 | 616, 618 | 9.54 ± 0.28 | ||
| 684 | 697 | 20.20 ± 1.33 | 684, 687 | 49.12 ± 1.27 | 0 | ||
| 733 | 744 | 1.60 ± 0.07 | 1.82 ± 0.02 | 0 | |||
| 733 | 748 | 0 | 103.06 ± 2.97 | 0 | |||
| 769 | 785 | 773, 774, 776, 778, 779, 780, 781, 782, 784 | 1.15 ± 0.10 | 772, 774, 781, 782, 783, 784 | 0.64 ± 0.00 | 772, 774, 776, 777, 780, 782 | 0.14 ± 0.02 |
| 786 | 806 | 0 | 17.55 ± 6.35 | 0 | |||
| 789 | 806 | pnf | 22.31 ± 0.31 | 0 | |||
| 789 | 809 | 2.67 ± 1.00 | 20.08 ± 0.15 | 0 | |||
| 813 | 831 | pnf | 819, 821, 822, 823 | 41.89 ± 0.18 | 0 | ||
| 843 | 857 | 0 | 843, 850, 853, 855 | 3.77 ± 0.14 | 843, 845, 846, 848, 849, 850, 852, 857 | 6.12 ± 4.49 | |