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. 2020 Jan 3;295(6):1754–1766. doi: 10.1074/jbc.RA119.011089

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Figure 5. — 14-3-3 protects Ser-99 phosphorylation of TET2 in vitro. A, schematic representation of the workflow for phosphatase protection assay. B, representative Western blot analysis of Myc-TET2 immunoprecipitates following in vitro R18 treatment. C, densitometry ratios of phosphorylated TET2/total Myc-TET2 (left) and total 14-3-3/total Myc-TET2 (right) were calculated from three biological replicates and plotted. Data were analyzed by one-way ANOVA followed by post hoc Tukey's honestly significant difference test. Left, ANOVA p value is 0.0146 (F = 9.2774). Post hoc analysis p values are demonstrated. Right, ANOVA p value is 0.0044 (F = 15.2941). Post hoc analysis p values are demonstrated. Error bars, S.E.