FIGURE 8.

Inhibition of carbachol (CCh)-induced increase in intracellular Ca²⁺ by MSG and MSG plus IMP in isolated muscle cells. Isolated gastric SMCs were loaded with 5 μmol/L fura-2 and treated with 10 μmol/L CCh before and after pretreatment with MSG (100 μmol/L) alone or MSG plus IMP (1 μmol/L). The SMCs were alternately excited at 380 nm and 340 nm. The background and autofluorescence were corrected from images of a SMC without the fura-2. Results in the representative tracing are expressed as 340/380 ratio and an increase in ratio reflects an increase in cytosolic Ca²⁺