Table 1.
TD-immunoassay and conventional ELISA for different concentrations of PCT spiked in sera
| PCT Concentration (pg/mL) |
TD-immunoassay* (pg/mL) (This work) |
Recovery (%) |
Replicates | Conventional ELISA (pg/mL) |
Recovery (%) |
Replicates |
|---|---|---|---|---|---|---|
| 1.9 | 1.8±0.3 | 95.7±19.7 | 3 | Not quantifiable | Not quantifiable | 3 |
| 3.9 | 4.0±0.5 | 103.4±11.8 | 3 | Not quantifiable | Not quantifiable | 3 |
| 7.8 | 8.6±0.5 | 110.7±7.2 | 3 | Not quantifiable | Not quantifiable | 3 |
| 20 | 21.4±0.4 | 107.0±2.1 | 3 | Not quantifiable | Not quantifiable | 3 |
| 31.3 | 36.3±4.0 | 115.9±12.8 | 3 | 29.6±6.5 | 94.6±14.3 | 3 |
| 200 | 215.7±30.0 | 95.5±15.0 | 3 | 197.6±7.8 | 98.5±3.9 | 3 |
| 1000 | 886.3±89.3 | 88.6±8.9 | 3 | 927±97.0 | 92.7±9.7 | 3 |
| 2000 | 2010.0±284.8 | 100.5±14.2 | 3 | 1923.2±114.4 | 96.2±5.7 | 3 |
| 6250 | 6132.2±790.5 | 98.1±12.6 | 3 | Not quantifiable | Not quantifiable | 3 |
| 12500 | 12463.1±1962 | 99.7±15.7 | 3 | Not quantifiable | Not quantifiable | 3 |
*
Results for TD-immunoassay were obtained the standard curve at 5 min (Supporting Information). Recovery (%) is the measured PCT concentration over the actual PCT concentration, which describes the accuracy of each technique.