Figure 2.

Fusarium virguliforme Pathogen Assays on Soybean and Maize.

(A) Plant growth and development of soybean and maize 14 DAI with F. virguliforme. Representative images show uninoculated (Mock) and F. virguliforme-inoculated (Inoc) plants. Bar = 4 cm.

(B) Trypan blue staining of inoculation sites on soybean cv Sloan and maize cv E13022S roots either inoculated with F. virguliforme or mock inoculated. Bars = 16 mm. Arrows point to areas of fungal growth and development; asterisks highlight appressoria-like structures.

(C) The percentage of unique RNA-seq reads aligned to F. virguliforme genome v2. Reads were trimmed by Trimmomatic v0.33 and aligned to Fusarium virguliforme genome v2 with HISAT2 v2.1.0. Each sample is indicated by a colored dot, and lines represent the mean of three biological replicates. Gray shade indicates SEM.