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. 2019 Dec 11;32(2):374–391. doi: 10.1105/tpc.19.00661

Figure 4.

Figure 4.

Nup96 Interacts with HOS1 in Plants.

(A) Coomassie Brilliant Blue staining of proteins immunoprecipitated by GFP-trap from the lysates of 35S:GFP wild-type (W) and Nup96-GFP nup96-1 (M) plants grown in long-day conditions for 9 d. Two replicates (Rep I and Rep II) were performed.

(B) Identification of the Nup96 complex by MS. HOS1 is highlighted by red color.

(C) Colocalization of Nup96-GFP and HOS1-mCherry in transgenic plant roots. Bars = 10 µm.

(D) BiFC assay showing the interaction of Nup96 and HOS1 in transgenic plants. BF, bright field. Bars = 20 µm.

(E) Co-IP assay showing the in vivo interaction of Nup96 and HOS1 in transgenic plants. GFP-trap was used to isolate Nup96-GFP from plant protein extracts, and HOS1-myc was then detected in the immunoprecipitates by anti-myc antibodies. HSP90 was used as the loading control for the input samples.