Figure 8.

Salt Stress–Induced KOR1 Internalization from the PM Is Distinct from Normal PM/TGN Cycling.

(A) Effect of drug treatment on the internalization of GFP-KOR1 under salt stress: Wm, a PI3K/PI4K inhibitor; LY294002, a PI3K inhibitor; PAO, a PI4K inhibitor; 1-butanol (1-ButOH), a PLD inhibitor; tert-butanol (tert-ButOH), negative control for 1-butanol. Five-day-old plants growing on a cellophane membrane placed on medium containing 1× MS salts and 3% (w/v) Suc were transferred to the same medium supplemented with 140 mM NaCl and/or inhibitors as indicated for 4 h. Bar = 10 µm.

(B) Quantitative data of (A). For each cell, the signal intensity at the PM was divided by the intracellular signal intensity. Error bars represent the se of the mean (n = 50). Different letters show significant differences between genotypes (P < 0.05, one-way ANOVA followed by Tukey’s honestly significant difference post hoc test).