Figure 5.

EUI Functions Downstream of OsDSK2a in GA Metabolism during the Seedling Stage.

(A) Growth of 10-d-old eui seedlings. eui-1 is a retrotransposon insertion mutant in the Zhenshan 97 (ZS97) background. eui-2 is a site mutant with a mutation at the 500th amino acid (Gly to Asp) in the BSM background.

(B) and (C) Lengths and fresh weights of the shoots shown in (A). The data represent mean ± sd (n = 15, *P ≤ 0.05, **P ≤ 0.01, Student’s t test).

(D) Contents of bioactive GAs and their precursors in 10-d-old eui seedlings. The data represent mean ± sd (n = 3).

(E) GA mediates the reduced growth of osdsk2a and enhanced growth of eui-1. Germinated DJ, osdsk2a, ZS97, and eui-1 seeds were incubated in standard liquid medium or medium supplemented with 10 μM PAC and different concentrations of GA₃ (0, 1, 10, and 100 μM) for 10 d. Bar = 2 cm.

(F) and (G) Lengths and fresh weights of the shoots shown in (E). The data represent mean ± sd (n = 15).

(H) Contents of bioactive GAs and their precursors in osdsk2a and the osdsk2a eui double mutant. n.d., not detected. The data represent mean ± sd (n = 3).

(I) Seedling growth of osdsk2a eui. Bar = 2 cm.

(J) and (K) Lengths and fresh weights of the shoots shown in (I). The data represent mean ± sd (n = 15, *P ≤ 0.05, **P ≤ 0.01, Student’s t test).