Figure 3.

R. mucilaginosa JGTA-S1 Improves N Metabolism in Rice.

(A) and (B) Rice plants were grown under N-replete conditions and treated with or without JGTA-S1. RNA was isolated from root and shoot tissue at 3 wpi and used for RT-qPCR. Expression levels of N-uptake and -assimilation genes at 3 wpi in (A) roots and (B) shoots.

(C) Expression levels of rice NIN-like transcription factor genes at 3 wpi.

(D) OrthoMCL clustering of rice NLP genes.

(E) Total N content per plant grown under N-replete conditions, as measured by the Kjeldahl method.

(F) to (H) Rice plants grown on artificial soil without N supplementation (low-N conditions). Effect of JGTA-S1 supplementation on rice was monitored compared with the untreated control after 10 DAI. (F) Digital image of rice plants. Shoot length (G) and chlorophyll content (H). Statistical analysis was performed using Mann-Whitney unpaired two-tailed t test. Data are given as mean ± se.