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. Author manuscript; available in PMC: 2020 Dec 19.
Published in final edited form as: Cell Chem Biol. 2019 Nov 6;26(12):1743–1754.e9. doi: 10.1016/j.chembiol.2019.10.008

Figure 3. Characterization of C321.ΔA.759 T7RNAP-Expressing Variants.

Figure 3.

(A) Characterization of the six C321.ΔA.759 T7RNAP-expressing variants generated in this study. Extracts derived from C321.ΔA.759 and its T7RNAP-expressing derivatives were directed to synthesize sfGFP in CFPS both with and without supplementation with purified T7RNAP, and fluorescence was measured after incubation for 20 h at 30°C. Three independent CFPS reactions were performed for each condition, and one standard deviation is shown.

(B) α-His western blot characterization of C321.ΔA.759.T7. pre: samples derived from cells immediately prior to induction. harv: samples derived from mid-exponential phase cells immediately prior to harvest. lys: samples derived from final clarified lysate.