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A side-by-side comparison of sfGFP produced in CFPS using crude lysates derived from IPTG-induced BL21 Star (DE3), C321.ΔA.759, C321.ΔA.759.T7, C321.ΔA.759.T7.D, and 759.T7.Opt cells. Shown are results from CFPS reactions performed both with and without supplementation with purified T7RNAP. At least three independent reactions were performed per condition, and one standard deviation is shown.
