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. 2020 Feb 3;8:26. doi: 10.3389/fbioe.2020.00026

TABLE 1.

Properties of DTEase family enzymes.

Optimum Molecular Optimal Metal Half-lifea Equilibrium kcat/Kmc
Sources of DTEase family enzymes temperature weights pH ions (min) ratiob (min–1mM–1)
Paenibacillus senegalensis (Yang et al., 2019) 55°C 33.5 8.0 Mn2+ 30:70 39
Caballeronia fortuita (Li et al., 2019) 65°C 33 7.5 Co2+ 63 37.5:62.5
Sinorhizobium sp. (Zhu et al., 2019b) 50°C 8.0 Mn2+
Staphylococcus aureus (Zhu et al., 2019a) 70°C 134.13 (tetramer) 8.0 Mg2+
Grobacterium sp. ATCC 31749 (Tseng et al., 2018) 55–60°C 32 7.5–8.0 Co2+ 267 (55°C) 30:70
Arthrobacter globiformis M30 (Yoshihara et al., 2017) 70°C 128 (tetramer) 7.0–8.0 Mn2+
Flavonifractor plautii (Park et al., 2016a) 65°C 33 7.0 Co2+ 130 156
Bacillus subtilis (He et al., 2016) 55°C 7.5–8.0 170
T. primitia ZAS-1 (Zhang et al., 2016b) 70°C 8.0 Co2+ ∼30 28:72(70°C) 144
Dorea sp. CAG317 (Zhang et al., 2015, 2018) 70°C 33 6.0 Co2+ ∼30 30:70(70°C) 412
C. bolteae (Jia et al., 2014) 55°C 139 (tetramer) 7.0 Co2+ 156 32:68(60°C) 107
Clostridium sp. (Mu et al., 2013) 65°C 130 (tetramer) 8.0 Co2+ 15 28:72 141.4
C. scindens 35.704 (Zhang et al., 2013a) 60°C 132 (tetramer) 7.5 Mn2+ 108 28:72(50°C) 64.5
Ruminococcus sp. (Zhu et al., 2012) 60°C 132 (tetramer) 7.5–8.0 Mn2+ 96 28:72 51
Clostridium cellulolyticum (Mu et al., 2011) 55°C 132 (tetramer) 8.0 Co2+ 408 32:68 186.4
Rhodobacter sphaeroides (Zhang et al., 2008) 60°C 64 (dimer) 9.0 Mn2+ 23:77
Agrobacterium Tumefaciens (Kim H.J. et al., 2006) 50°C 132 (tetramer) 8.0 Mn2+ 64 33:67 205
Pseudomonas cichorii (Itoh et al., 1994; Ishida et al., 1997a) 40°C, 60°C 68 (dimer) 7.5 20:80
Bacillus subtilis (Patel et al., 2019) 60°C, 70°C 6–11 Co2+ Mn2+ 28:72

a,b,cRepresent the optimum parameters of temperature, substrate, and pH, respectively. – Means not evaluated.