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A) Image of the Location toolbox within the Mosaic Targeted Illumination device setting. Coordinate system setting is to 60x Zyla, with target location set to the Active Region. B) Time-lapse toolbox within the Mosaic Targeted Illumination device setting showing the number of timepoints set to 100 at 30 second intervals with pulses before timepoints 10–13. A journal is used to image the 561 and 647 channels during each of these timepoints.
