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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Curr Protoc Cell Biol. 2020 Mar;86(1):e102. doi: 10.1002/cpcb.102
Reagent 1500ul total 1000ul total 800ul total 500ul total 300ul total
Col1 840.3ul 560.2ul 448.2ul 280.1ul 168.1ul
NaHCO3 35.8ul 23.8ul 19.1ul 11.9ul 7.2ul
HEPES 30ul 20ul 16ul 10ul 6ul
DMEM 593.9ul 395.9ul 316.7ul 198ul 118.8ul