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. 2020 Feb 3;10:46. doi: 10.3389/fonc.2020.00046

Figure 2.

Figure 2

Cav-1 attenuates apoptotic response induced by cisplatin in GC cells. (A) Overexpression of Cav-1 inhibited the apoptosis of AGS cells. (B) Statistical analysis of the apoptotic rate of AGS cell population after CDDP treatment. (C) Knock-down of Cav-1 enhanced the apoptosis of MGC803 cells. (D) Statistical analysis of the apoptotic rate of MGC803 cell population after CDDP treatment. (E) The expression of cleaved caspase-3, cleaved caspase-9, and cleaved PARP were notably down-regulated in AGS/Cav-1+ cells compared with AGS/EV cells. (F) The relative levels of cleaved caspase-3, cleaved caspase-9, cleaved PARP and Cav-1 in AGS cells were analyzed. (G) Knock-down of Cav-1 substantially increased the levels of cleaved caspase-3, cleaved caspase-9, and cleaved PARP in MGC803/shCav-1 cells compared with MGC803/NC cells. (H) The relative levels of cleaved caspase-3, cleaved caspase-9, cleaved PARP, and Cav-1 in MGC803 cells were analyzed. Data are shown as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P <0.001 compared with AGS/EV cells or MGC803/NC cells. #P <0.05 compared with CDDP for 24 h after transfected with the empty or negative control vector. CDDP, cisplatin.