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. 2020 Jan 2;14(6):061005. doi: 10.1063/1.5126493

Fig. 5.

Fig. 5.

(a)–(f) Images taken on DIV 1 with a 10× objective lens to visualize RDF cell bodies (endogenous GFP, green), F-actin stress fibers (TRITC-phalloidin, red), and cell nuclei (DAPI, blue). (a) There is virtually no cell attachment to gels without grafted peptide. (b) Very few cells attached to hydrogels quenched with the negative control peptide PEGMA-SDGRG (0 mM RGD), and of the cells that adhered, none had spread. (c) RDFs partially spread on hydrogels quenched with a 1:3 ratio of the active ligand to the inactive peptide (∼0.8 mM RGD) and (d) RDFs exhibited stellate shapes on hydrogels quenched with the adhesive ligand PEGMA-GRGDS (∼3.2 mM RGD). (e) No significant differences were observed when a greater excess of the active ligand (9 mM) had been applied to the hydrogels. (f) RDF attachment and spreading on positive control hydrogels of 2.0 mg/mL type-I bovine collagen.