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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: J Cell Biochem. 2019 Jun 27;120(11):19004–19018. doi: 10.1002/jcb.29223

Figure 2: Effect of FS mutation on dsRNA-binding.

Figure 2:

dsRNA-binding activity of wt PACT and FS mutant was measured by poly(I)-poly(C)-agarose binding assay with in vitro translated 35S-labeled proteins. The positions of PACT and FS bands are indicated by arrows and the molecular weights are as indicated. T, total input; B, proteins bound to poly(I)-poly(C)-agarose. Competition lanes 5 and 6: competition with 100-fold molar excess of ssRNA (ss) or dsRNA (ds). The fainter bands below the parent PACT bands represent products of in vitro translation from internal methionine codons. The firefly luciferase, which does not bind dsRNA, was used as a negative control. B. Quantification of dsRNA-binding assay. Bands were quantified by phosphorimager analysis and % bound was calculated. Error bars: standard error of mean from 4 independent experiments. wt PACT: blue bar and FS mutant: red bar.