Fig. 8.

Primary microglia from four different genotypes were exposed to LPS (100 ng/ml) and IFN-γ (30 ng/ml) for 24 (a–c) or 18 h (d–f). a NO production was measured by the Griess reagent and normalized to the cell number in individual wells, as assessed by crystal violet staining. b, c TNF-α and IL-6 levels were evaluated by ELISA and normalized to the cell number in individual wells, as assessed by crystal violet staining. d–f Western blot analysis of the expression of Cox-2 and iNOS (d) and diagram of the normalized results for Cox-2 (e) and iNOS (f). The results are presented as mean ± SE; n = 4–6. The asterisks indicate statistical significance, *p value < 0.05; **p value < 0.01 for WT vs KL-OE and WT vs WT+rmKL, #p value < 0.05; ##p value < 0.01; ###p value < 0.001 for SOD1 vs KL-OE/SOD1 and SOD1 vs SOD1+ rmKL, as analyzed by one-way ANOVA with post hoc Tukey’s test