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. Author manuscript; available in PMC: 2020 Feb 10.
Published in final edited form as: Immunohorizons. 2019 Jul 17;3(7):331–340. doi: 10.4049/immunohorizons.1900033

FIGURE 1. Generation and initial characterization of mice lacking Ets1 in B cells.

FIGURE 1.

(A) Generation of B cell–specific Ets1 knockout mice. Diagram of the floxed allele (top) in which loxP sites flank the final two exons of the Ets1 gene (exons 7 and 8) and the deleted allele (bottom). (B) Western blot for Ets1 protein (top) using lysates from purified splenic B cells. GAPDH was used as a loading control (bottom). (C) Spleens from mice of the indicated genotypes showing that Ets1−/−, but not CD19-Cre Ets1fl/fl, mice have enlarged spleens. (D–F) Quantification of the number of total splenocytes (n = 20 Ets1+/+ and Ets1−/− and 12 CD19-Cre Ets1+/+ and CD19-Cre Ets1fl/fl mice), splenic B cells (n = 8 Ets1+/+, 6 Ets1−/−, 11 CD19-Cre Ets1+/+, and 12 CD19-Cre Ets1fl/fl mice), and splenic CD4+ T cells (n = 7 Ets1+/+, 5 Ets1−/−, 10 CD19-Cre Ets1+/+, and 8 CD19-Cre Ets1fl/fl mice) in mice of the indicated genotypes. **p < 0.01. ns, not significant.