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. Author manuscript; available in PMC: 2020 Feb 10.
Published in final edited form as: Immunohorizons. 2019 Jul 17;3(7):331–340. doi: 10.4049/immunohorizons.1900033

FIGURE 3. Mice with a B cell–specific deletion of Ets1 have increased memory phenotype B cells but no increase in switching to IgG1.

FIGURE 3.

(A) Flow cytometry analysis of PDL2 versus CD80 in gated live B220+ B cells showing that Ets1−/− and CD19-Cre Ets1fl/fl mice have increased percentages of B cells with a memory phenotype (PDL2+CD80+). (B) Quantification of the percentages of memory B cells in spleen and lymph nodes of the various strains of mice (n = 6 Ets1+/+, 6 Ets1−/−, 9 CD19-Cre Ets1+/+, and 6 CD19-Cre Ets1fl/fl mice). (C) Flow cytometry analysis of B220 versus IgG1 in gated live splenocytes showing that Ets1−/− mice, but not CD19-Cre Ets1fl/fl mice, have increased percentages of IgG1+ B cells. (D) Quantification of the percentages of IgG1+ B cells in the spleen and lymph nodes of the various strains of mice (n = 21 Ets1+/+, 17 Ets1−/−, 11 CD19-Cre Ets1+/+, and 12 CD19-Cre Ets1fl/fl mice). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ns, not significant.