Table 3.

Compositional data, intake values, reference points and margin of exposure (MOE) for the individual components of cardamom oil classified according to assessment groups based on chemical groups (CGs) as defined in Annex I of Regulation (EC) No 1565/2000a

Essential oil composition			Exposure		Hazard characterisation		Risk characterisation
Assessment group	FLAVIS No	Max conc. in the oil	Max Feed conc.	Daily Intake	Cramer Class	NOAELb	MOE	MOET
Constituent	–	%	mg/kg	mg/kg bw per day	–	mg/kg bw per day	–	–
CG 6
Terpineol acetate	09.830	37.3	1.865	0.1473	I	250	1,697
α‐Terpineol		2.03	0.118	0.0093	I	250	26,932
Linalyl acetate	09.013	7.01	0.351	0.0277	I	117	4,225
Linalool	02.013	4.62	0.231	0.0182	I	117	6,411
Nerolidol	02.018	1.56	0.078	0.0062	I	117	18,987
Terpinen‐4‐ol	02.072	2.03	0.102	0.0080	I	250	31,178
β‐Terpineol	02.097	■■■■■	0.043	0.0034	I	250	73,594
Dihydroterpinyl acetate	n.a.	■■■■■	0.035	0.0027	I	250	91,726
CG 6				0.2229				886
CG 16
1,8‐Cineole	03.001	24.13	1.207	0.0953	II	562.5	5,902
CG 31, II (Acyclic alkanes)
Myrcene	01.008	2.63	0.132	0.0104	I	44	4,235
CG 31, III (Cyclohexene hydrocarbons)
Limonene	01.045	4.7	0.235	0.0186	I	250	13,466
γ‐Terpinene	01.020	■■■■■	0.022	0.0017	I	250	147,189
α‐Terpinene	01.019	■■■■■	0.015	0.0011	I	250	218,245
Terpinolene	01.005	■■■■■	0.012	0.0009	I	250	263,713
β‐Bisabolene	01.028	■■■■■	0.047	0.0037	I	250	67,331
β‐Sesquiphellandrene	n.a.	■■■■■	0.019	0.0015	I	250	171,057
α‐Zingiberene	n.a.	■■■■■	0.023	0.0018	I	3	1,688
β‐Elemene	n.a.	■■■■■	0.019	0.0015	I	3	1,999
CG 31, III				0.0308				831
CG 31, IVe (Benzene hydrocarbons, alkyl)
p‐Cymene	01.002	■■■■■	0.080	0.0063	I	154	24,367
CG 31, V (Bi‐, tricyclic, non aromatic hydrocarbons)
α‐Pinene	01.004	2.76	0.138	0.0109	I	222	20,363
β‐Pinene	01.003	■■■■■	0.041	0.0032	I	222	69,386
Camphene	01.009	■■■■■	0.012	0.0009	I	222	234,177
β‐Caryophyllene	01.007	■■■■■	0.010	0.0008	I	222	295,803
Sabinene	01.059	4.78	0.239	0.0189	I	222	11,758
α‐Selinene	n.a.	■■■■■	0.016	0.0012	I	222	181,298
CG 31, V				0.0359				6,183
CG 31, VI (macrocyclic non aromatic hydrocarbons)
δ‐Germacrene	01.042	■■■■■	0.008	0.0006	I	3	5,063
β‐Germacrene	n.a.	■■■■■	0.008	0.0006	I	3	4,747
CG 31, VI				0.0012				2,450
CG 3
Geraniol	02.012	■■■■■	0.095	0.0075	I	345	45,969
Geranyl acetate	09.011	■■■■■	0.095	0.0075	I	345	46,213
Geranial	05.188	■■■■■	0.029	0.0023	I	345	153,231
Geranic acid	n.a.	■■■■■	0.010	0.0008	I	345	436,709
(Z)‐Nerol	02.058	■■■■■	0.015	0.0011	I	345	301,179
Farnesol	02.029	■■■■■	0.008	0.0006	I	1,000	1,582,278
CG 3				0.0198				17,806
CG 4
Citronellol	02.011	■■■■■	0.037	0.0029	I	50	17,106
CG 8
Bornyl acetate	09.017	■■■■■	0.025	0.0020	I	3	1,519
Fenchyl alcohol c	02.038	■■■■■	0.019	0.0015	I	3	1,999
trans‐Sabinene hydrate	02.085	■■■■■	0.099	0.0078	I	222	28,529
CG 8				0.0113				838
CG 1
Octyl acetate	09.007	■■■■■	0.060	0.0047	I	120	25,316
Decanoic acid	08.011	■■■■■	0.120	0.0095	I	1,000	105,485
CG 1				0.0142				20,416

FLAVIS: the EU Flavour Information System; bw: body weight; NOAEL: no observed adverse effect level; MOE: margin of exposure; CG: chemical group.

^aIntake calculations for the individual components are based on the use level of 5 mg/kg in feed for chickens for fattening, the species with the highest ratio of feed intake/body weight. The MOE for each component is calculated as the ratio of the reference point (NOAEL) to the intake. The combined margin of exposure (MOET) is calculated for each assessment group as the reciprocal of the sum of the reciprocals of the MOE of the individual substances.

^bvalues in bold refer to those components for which the NOAEL value was available, values in italics are the 5th percentile of the distribution of NOAELs of the corresponding Cramer Class, other values (plain text) are NOAELs extrapolated by using read‐across.

^cor isobornyl acetate.