Table D.1.

Genotoxicity data (in vitro)

Chemical name [FL‐no]	Test system	Test object	Concentration	Reported result	Reference	Commentsc
α‐Ionone [07.007]	Chromosomal aberration	Chinese hamster B241 cell line	25 nmol/L	Positivea	Kasamaki et al. (1982)	Limited validity (limited documentation; results for only one test concentration reported; long incubation period of 24 h; unusual cell line)
	Reverse mutation	S. typhimurium TA98, TA100	0.01–50 μg/plate	Negativea	Kasamaki et al. (1982)	Limited validity (insufficiently reported; only two strains)
	Rec assay	B. subtilis H17 & M45	19 mg/disc	Negativeb	Oda et al. (1978)	Insufficient validity. This bacterial DNA‐repair test system is of low predictive value for genotoxicity
Methyl‐α‐ionone [07.009]	Reverse mutation	S. typhimurium TA1535, TA1537, TA1538, TA98, TA100	5 concentrations up to cytotoxicity or max 3,600 μg/plate	Negativea	Wild et al. (1983)	Limited validity (no TA102 or E. coli)
Methyl‐δ‐ionone [07.088]	Reverse mutation	S. typhimurium TA1535, TA1537, TA1538, TA98, TA100	5 concentrations up to cytotoxicity or max 3,600 μg/plate	Negativea	Wild et al. (1983)	Limited validity (no TA102 or E. coli)

^aWith and without metabolic activation.

^bActivation status unknown.

^c

Validity of genotoxicity studies:

• Valid.
• Limited validity (e.g. if certain aspects are not in accordance with OECD Guidelines or current standards and/or limited documentation).
• Insufficient validity (e.g. if main aspects are not in accordance with any recognised guidelines (e.g. OECD) or current standards and/or inappropriate test system).
• Validity cannot be evaluated (e.g. insufficient documentation, short abstract only, too little experimental details provided).