Table 1.
Reference methods listed by BVL (2018) and available standard methods
| E number(s) | Method number, name, origin | Analyte(s) | Analytical technique | Matrices |
|---|---|---|---|---|
| BVL methods | ||||
| 450–452 | L 06.00‐15L 07.00‐20L 08.00‐22 | Condensed phosphates | Thin‐layer chromatography | Meat, meat products, processed meats, bakery wares |
| 450–452 | L 06.00‐9 | Di‐, Tri‐, Poly‐ Phosphate | Spectrophotometry | Foodstuff, e.g. meat products, fish products, dairy, bakery products, grain‐based foods |
| 450(i–vii) | L 06.00‐15ISO‐Norm 5553 | Diphosphate | Thin‐layer chromatography | Dairy, meat products, fish products |
| 451(i, ii) | L 06.00‐15ISO‐Norm 5553 | Triphosphate | Thin‐layer chromatography | Dairy, meat products, fish products |
| 452(i–iv) | L 06.00‐15 mod. Iso‐Norm 5553(qualitative)L 06.00‐09 mod. (quantitative) | Di‐, Tri‐, Poly‐ Phosphate | Thin‐layer chromatography Spectrophotometry | Meat products, dairy (cheese, processed cheese), fish products |
| 338–341, 343,450–452 | Photometric determination of phosphate after acid digestion in drinks | Total phosphate as PO4 | Spectrophotometry | Soft drinks |
| 338–341, 343,450–452 | L 06.00‐9 | Total phosphate as P2O5 | Spectrophotometry | Meat, meat products, cheese, dairy |
| 338–343, 450, 451 | Condensed phosphatesL 06.00‐15 | Condensed phosphates | Qualitative chromatography | Meat and meat products |
| 338–343, 450, 451 | Total phosphorus contentL 06.00‐9 | P2O5 | Spectrophotometry | Meat and meat products |
| 338–343, 450, 451 | Total phosphorus contentL 03.00‐17 | Phosphorous | Spectrophotometry | Cheese, processed cheese, processed cheese preparations |
| 339(i–iii) | L 06.00‐15 mod | Triphosphate | Thin‐layer chromatography | Fish products |
| 340(i–iii) | Not specified | Phosphoric acid | Ion chromatography | Soft drinks |
| 338 | L 31.00‐6 | Phosphate | Spectrophotometry without ashing | Soft drinks |
| Other standard methods/norms | ||||
| BSI 4401‐15:1981/ISO 5553;1981. Methods of test for meat and meat products. Detection of polyphosphates (by spectrophotometry) | ||||
| PD ISO/TS 18083:2013. Processed cheese products. Calculation of content of added phosphate calculated as phosphorus (by spectrophotometry) | ||||
| AOAC, 1997. Standard colorimetric method for the determination of orthophosphate in water (by spectrophotometry) | ||||