Table 5.

Ecotoxicity studies required in Phase IIA to derive PNEC_I;soil

Study	Toxicity endpoint	AF	Remark
Nitrogen Transformation (28 days), OECD 216.	≤ 25% of control	1	Exposure 1X and 10X PECmax
Terrestrial plants (14–21 days), OECD 208.	EC50	100	The most sensitive endpoint (emergence, biomass or height of sprout) of all plant species tested
Earthworm acute (14 days), OECD 207.	LC50	1,000	–

AF: assessment factor; EC₅₀: concentration of the additive causing effect in 50% of the population the most sensitive OECD endpoint; LC₅₀: concentration of the additive that kills 50% of the population.