Table 6.
: Ecotoxicity studies required in Phase IIA to derive PNECI;sw
| Study | Toxicity endpoint | AF | Remark |
|---|---|---|---|
| Algal growth inhibitiona, OECD 201. | 72‐h ErC50 b | 1,000 | EyC50 c may be used if ErC50 not reported |
| Daphnia immobilization, OECD 202. | 48‐h EC50 | 1,000 | – |
| Fish acute toxicity, OECD 203. | 96‐h LC50 | 1,000 | – |
a
In case problems arise with coloured additives, Lemna ( https://www.oecd-ilibrary.org/environment/test-no-221-lemna-sp-growth-inhabition-test_9789264016194-en) can be used.
b
ErC50: the concentration of test substance which results in a 50 percent reduction in growth rate.
c
EyC50: the concentration of the test substance with results in a 50% reduction of yield.