Table A.1.

Overview of relevant aquatic SPU organisms, examples of related standard test species and current (2017) basic data requirements in the EFSA FEEDAP 2008 ERA guidance for feed additives

SPU‐Organism group	Examples of standard test species/assays	Phase II data requirements Feed additives
Aquatic microbes	OECD test on inhibition of anaerobic bacteria in sludge or sediment; ISO test on inhibition of nitrification in activated sludge	No
Aquatic Protozoa	Currently no official OECD Test Guideline available (the freshwater protozoan Tetrahymena pyriformis and the marine protozoan Uronema marinum may be good candidates for guideline development)	No
Algae	OECD tests with algae (e.g. Pseudokirchneriella subcapitata)	Yes
Aquatic macrophytes	OECD tests with Lemna sp. and Myriophyllum spicatum	No
Aquatic arthropods	OECD tests with Daphnia sp. and Chironomus sp.; ASTM test with Hyalella azteca, Diporeia spp., Leptocheirus plumulosus, Eohaustorius estuarius, Ampelisca abdita, Rhepoxynius abronius and Hexagonis spp.; ISO test with Corophium volutator	Yes, for freshwater ecosystems Daphnia magna and a sediment‐dwelling organism (e.g. Chironomus) Yes for marine sediment–dwelling taxa (e.g. Leptocheirus, Ampelisca, Rhepoxynius and Corophium)
Other invertebrates	OECD test with Lumbriculus variegatus; ISO test with Caenorhabditis elegans; ASTM test with Neanthes arenaceodentata	Yes, for freshwater ecosystems a sediment‐dwelling organism (e.g. Lumbriculus or Caenorhabditis) Yes for marine sediment‐dwelling taxa (e.g. Neanthes)
Aquatic vertebrates	OECD test with Oncorhynchus mykiss; ASTM test with Rana pipiens	Yes for freshwater fish