. 2018 Jul 25;16(7):e05349. doi: 10.2903/j.efsa.2018.5349

Table 2.

Genetic elements in the expression cassettes of the events stacked in cotton GHB614 × T304‐40 × GHB119

Event	Promoter	5’ UTR	Transit peptide	Coding region	Terminator
GHB614	Ph4a748At (histone H4) (Arabidopsis thaliana)	First intron of gene II of histone H3.III variant (A. thaliana)	Optimized tp (Zea mays and Helianthus annuus)	2mepsps (Zea mays)	3’ histone H4 (A. thaliana)
T304‐40	Ps7s7 (Subterranean clover stunt virus genome segment 7) 35S (Cauliflower mosaic virus P35S3)	Tapetum‐specific E1 (5’ e1 from Oryza sativa)	–	cry1Ab (Bacillus thuringiensis, subsp berliner 1715) bar (Streptomyces hygroscopicus)	3’ NADP‐me1 (Flaveria bidentis) 3’ nos (A. tumefaciens)
GHB119	35S (Cauliflower Mosaic virus) Pcsvmv XYZ (Cassava Vein Mosaic Virus)	5’ cab22L (Petunia hybrida)	TpssuAt (ats1A gene of A. thaliana) –	cry2Ae (Bacillus thuringiensis subsp. dakota 1715) bar (Streptomyces hygroscopicus strain ATCC21705)	3’ 35S (Cauliflower Mosaic virus) 3’ nos (A. tumefaciens)

Event

Promoter

5’ UTR

Transit peptide

Coding region

Terminator

GHB614

Ph4a748At (histone H4)

(Arabidopsis thaliana)

First intron of gene II of histone H3.III variant (A. thaliana)

Optimized tp (Zea mays and Helianthus annuus)

2mepsps (Zea mays)

3’ histone H4 (A. thaliana)

T304‐40

Ps7s7 (Subterranean clover stunt virus genome segment 7)

35S (Cauliflower mosaic virus P35S3)

Tapetum‐specific E1 (5’ e1 from Oryza sativa)

–

cry1Ab (Bacillus thuringiensis, subsp berliner 1715)

bar (Streptomyces hygroscopicus)

3’ NADP‐me1 (Flaveria bidentis)

3’ nos (A. tumefaciens)

GHB119

35S (Cauliflower Mosaic virus)

Pcsvmv XYZ (Cassava Vein Mosaic Virus)

5’ cab22L (Petunia hybrida)

TpssuAt (ats1A gene of A. thaliana)

–

cry2Ae (Bacillus thuringiensis subsp. dakota 1715)

bar (Streptomyces hygroscopicus strain ATCC21705)

3’ 35S (Cauliflower Mosaic virus)

3’ nos (A. tumefaciens)

–: when no element was specifically introduced to optimise expression.