Means, standard deviation and ranges of protein levels (μg/g dry weight) from cotton GHB614 × T304‐40 × GHB119 (not treated), GHB614 (not treated), T304‐40 (not treated) and GHB119 (not treated) from field trials performed in USA in 2013a
| GHB614 × T304‐40 × GHB119 | GHB614 | T304‐40 | GHB119 | |
|---|---|---|---|---|
| PAT | ||||
| Leaf (4–6 leaf) |
(452.46–678.41)d |
366.50 ± 55.85 (292.28–455.13) |
232.65 ± 34.16 (178.30–293.36) |
|
|
Leaf (square initiation) |
1366.02 ± 481.35 (852.22–2164.34) |
663.13 ± 56.75 (553.32–746.27) |
380.83 ± 73.21 (294.73–512.23) |
|
|
Leaf (2 weeks after first flower) |
770.37 ± 183.41 (478.33–1083.54) |
372.36 ± 101.55 (220.05–552.15) |
263.23 ± 66.97 (174.27–399.16) |
|
| Root (4–6 leaf) |
247.88 ± 64.45 (160.15–361.73) |
146.11 ± 17.82 (115.87–167.46) |
88.32 ± 19.56 (34.90–105.17) |
|
| Pollene (flowering) |
1.12 ± 0.68 (0.50–2.30) |
0.75 ± 1.00 (0.03–3.31) |
1.53 ± 1.00 (0.29–3.06) |
|
|
Squares (2 weeks after first flower) |
1036.73 ± 169.99 (803.85–1356.51) |
536.98 ± 195.59 (172.65–852.53) |
323.46 ± 34.84 (277.09–396.89) |
|
|
Bolls (2 weeks after first flower) |
364.71 ± 34.77 (313.85–420.69) |
220.55 ± 18.50 (196.41–251.86) |
161.64 ± 32.09 (131.84–246.23) |
|
|
Whole plant (2 weeks after first flower) |
489.07 ± 80.88 (345.18–650.41) |
322.14 ± 115.35 (147.96–586.87) |
165.98 ± 14.14 (142.94–190.56) |
|
| Fuzzy seed (maturity) |
216.17 ± 23.28 (175.51–248.65) |
110.95 ± 12.80 (85.41–130.41) |
96.10 ± 12.37 (74.80–117.54) |
|
| Cry1Ab | GHB614 × T304‐40 × GHB119 | GHB614 | T304‐40 | GHB119 |
| Leaf (4–6 leaf) |
16.74 ± 6.77 (7.42–24.52) |
15.15 ± 6.58 (6.90–23.01) |
||
| Leaf (square initiation) |
27.41 ± 12.92 (9.80–47.12) |
20.03 ± 8.90 (10.81–37.33) |
||
|
Leaf (2 weeks after first flower) |
10.06 ± 4.16 (2.31–15.46) |
7.87 ± 4.05 (1.93–13.92) |
||
| Root (4–6 leaf) |
12.13 ± 2.43 (8.03–16.39) |
11.36 ± 2.13 (8.98–16.17) |
||
| Pollen (flowering) |
0.20 ± 0.11 (0.09–0.41) |
0.35 ± 0.23 0.12–0.82 |
||
|
Squares (2 weeks after first flower) |
13.13 ± 3.48 (9.82–19.02) |
9.93 ± 3.94 (4.87–16.78) |
||
|
Bolls (2 weeks after first flower) |
10.81 ± 2.57 (7.25–14.36) |
9.38 ± 1.82 (6.37–12.87) |
||
|
Whole plant (2 weeks after first flower) |
9.71 ± 1.76 (7.05–11.92) |
10.07 ± 4.63 (5.98–22.19) |
||
| Fuzzy seed (maturity) |
6.37 ± 1.80 (4.07–9.34) |
6.56 ± 1.25 (5.24–9.05) |
||
| 2mEPSPS | GHB614 × T304‐40 × GHB119 | GHB614 | T304‐40 | GHB119 |
| Leaf (4–6 leaf) |
410.37 ± 63.16 (297.54–497.53) |
405.31 ± 50.37 (317.74–480.82) |
||
|
Leaf (square initiation) |
1912.52 ± 676.00 (876.48–2572.18) |
1540.20 ± 767.54 (495.63–2790.01) |
||
|
Leaf (2 weeks after first flower) |
494.21 ± 128.44 (303.34–749.60) |
382.68 ± 48.55 (287.74–475.37) |
||
| Root (4–6 leaf) |
86.27 ± 18.46 (54.05–109.59) |
85.62 ± 29.03 (22.19–123.87) |
||
| Pollen (flowering) |
8.24 ± 3.19 (5.32–14.90) |
8.95 ± 6.69 (2.43–23.14) |
||
|
Squares (2 weeks after first flower) |
390.51 ± 78.33 (267.43–499.86) |
393.71 ± 101.61 (251.09–538.67) |
||
|
Bolls (2 weeks after first flower) |
143.35 ± 21.32 (110.39–177.56) |
158.84 ± 23.38 (126.42–195.79) |
||
|
Whole plant (2 weeks after first flower) |
216.86 ± 45.19 (155.57–311.10) |
253.92 ± 161.81 (171.84–754.31) |
||
| Fuzzy seed (maturity) |
154.57 ± 20.89 (128.93–192.99) |
159.36 ± 13.06 (131.33–180.65) |
||
| Cry2Ae | GHB614 × T304‐40 × GHB119 | GHB614 | T304‐40 | GHB119 |
| Leaf (4–6 leaf) |
172.41 ± 54.07 (102.09–263.04) |
190.74 ± 51.99 (95.49–268.24) |
||
|
Leaf (square initiation) |
226.52 ± 44.18 (165.69–305.99) |
218.94 ± 55.07 (157.80–324.63) |
||
|
Leaf (2 weeks after first flower) |
138.84 ± 27.59 (96.10–202.71) |
119.66 ± 37.03 (78.62–207.88) |
||
| Root (4–6 leaf) |
10.81 ± 5.91 (3.64–21.95) |
12.19 ± 2.90 (4.62–16.44) |
||
| Pollen (flowering) |
0.21 ± 0.14 (0.04–0.58) |
0.28 ± 0.41 (0.06–1.56) |
||
|
Squares (2 weeks after first flower) |
42.75 ± 13.09 (30.96–63.72) |
42.16 ± 14.95 (26.80–69.75) |
||
|
Bolls (2 weeks after first flower) |
19.54 ± 4.10 (13.58–24.75) |
23.99 ± 6.52 (14.91–38.02) |
||
|
Whole plant (2 weeks after first flower) |
111.81 ± 31.51 (79.15–173.47) |
135.11 ± 46.13 (78.92–235.72) |
||
| Fuzzy seed (maturity) |
29.15 ± 6.14 (19.10–39.14) |
26.79 ± 7.53 (15.00–35.10) |
||
Number of samples is n = 12 except for: PAT in leaf at square initiation and fuzzy seed (n = 11 for GHB614 × T304‐40 × GHB119); 2mEPSPS in leaf at square initiation and fuzzy seed (n = 11 for GHB614 × T304‐40 × GHB119) and squares (n = 11 for GHB614); Cry1Ab in fuzzy seed (n = 11 for GHB614 × T304‐40 × GHB119); Cry2Ae in leaf/square initiation and fuzzy seed (n = 11 for GHB614 × T304‐40 × GHB119).
Mean.
Standard deviation.
Range.
Reported values for pollen are derived from fresh weight (FW) tissue.