Table 76.
Overview of results using RT‐PCR screening for carbapenemase‐resistant Enterobacteriaceae in faecal samples of livestock in 2015 and 2016 in the Netherlands
| Animal species | Number of samples screened for CPE | Number of samples positive for CPE | Prevalence (%) (95% IC) |
|---|---|---|---|
| Broilers | 1,000 | 0 | 0 (0–0.37) |
| Layers | 193 | 0 | 0 (0–1.89) |
| Ducks | 100 | 0 | 0 (0–3.62) |
| Dairy cattle | 602 | 0 | 0 (0–0.61) |
| Veal calves | 605 | 0 | 0 (0–0.61) |
| Slaughter pigs | 600 | 0 | 0 (0–0.61) |
Comment provided by the Netherlands: Wang et al. described a PCR‐based method to detect bla NDM directly from faecal samples and compared the results to culture diagnostics (Wang, 2017). They demonstrated that bla NDM was present in 165/330 (50%) samples from commercial farms, whereas culture‐based methods detected carbapenemase‐resistant Enterobacteriaceae (CRE) in 83/330 (25%) samples only.
Table 76.
Overview of results using RT‐PCR screening for carbapenemase‐resistant Enterobacteriaceae in faecal samples of livestock in 2015 and 2016 in the Netherlands
| Animal species | Number of samples screened for CPE | Number of samples positive for CPE | Prevalence (%) (95% IC) |
|---|---|---|---|
| Broilers | 1,000 | 0 | 0 (0–0.37) |
| Layers | 193 | 0 | 0 (0–1.89) |
| Ducks | 100 | 0 | 0 (0–3.62) |
| Dairy cattle | 602 | 0 | 0 (0–0.61) |
| Veal calves | 605 | 0 | 0 (0–0.61) |
| Slaughter pigs | 600 | 0 | 0 (0–0.61) |
Comment provided by the Netherlands: Wang et al. described a PCR‐based method to detect bla NDM directly from faecal samples and compared the results to culture diagnostics (Wang, 2017). They demonstrated that bla NDM was present in 165/330 (50%) samples from commercial farms, whereas culture‐based methods detected carbapenemase‐resistant Enterobacteriaceae (CRE) in 83/330 (25%) samples only.