Table A.1.
Means, standard deviation and ranges of protein levels (μg/g dry weight) from cotton GHB614 × LLCotton25 × MON 15985 (treated with glyphosate and glufosinate), GHB614 (treated with glyphosate), LLCotton25 (treated with glufosinate), and MON 15985 (not treated), from field trials performed in USA in 2014a
| GHB614 × LLCotton25 × MON 15985 | GHB614 | LLCotton25 | MON 15985 | |
|---|---|---|---|---|
| Cry1Ac | ||||
| Leaf (4–6 leaf) | 32.22b ± 9.85c (21.19–54.12)d | 31.90 ± 8.38 (15.72–47.80) | ||
| Leaf (square initiation) | 31.00 ± 15.85 (14.79–64.51) | 27.59 ± 8.66 (16.79–45.74) | ||
| Leaf (2 weeks after first flower) | 25.37 ± 7.20 (14.93–34.45) | 22.55 ± 6.35 (16.16–40.31) | ||
| Root (square initiation) | 4.04 ± 0.64 (< LLOQ–5.01) | 5.25 ± 1.64 (< LLOQ–7.84) | ||
| Pollen (flowering)e | 0.21 ± 0.05 (0.15–0.34) | 0.18 ± 0.02 (0.14–0.21) | ||
| Fuzzy seed (maturity) | 11.35 ± 1.13 (9.92–13.22) | 10.14 ± 1.50 (7.13–11.96) | ||
| Cry2Ab2 | GHB614 × LLCotton25 × MON 15985 | GHB614 | LLCotton25 | MON 15985 |
| Leaf (4–6 leaf) | 167.35 ± 36.91(121.95–255.62) | 139.60 ± 20.86(103.94–175.15) | ||
| Leaf (square initiation) | 156.29 ± 32.36 (111.96–203.41) | 182.70 ± 20.21 (140.73–218.23) | ||
| Leaf (2 weeks after first flower) | 180.57 ± 26.88 (140.52–232.73) | 189.77 ± 16.09 (164.39–221.56) | ||
| Root (square initiation) | 78.71 ± 15.84 (47.63–101.15) | 84.39 ± 13.06 (62.19–98.64) | ||
| Pollen (flowering) | 0.90 ± 0.75 < LLOQ–2.88 | 0.80 ± 0.51 (< LLOQ–2.27) | ||
| Fuzzy seed (maturity) | 171.21 ± 24.03 (136.07–215.60) | 152.77 ± 18.24 (122.26–181.50) | ||
| 2mEPSPS | GHB614 × LLCotton25 × MON 15985 | GHB614 | LLCotton25 | MON 15985 |
| Leaf (4–6 leaf) | 279.67 ± 125.83 (104.87–453.86) | 303.66±78.10 (207.34–470.87) | ||
| Leaf (square initiation) | 507.11 ± 188.84 (234.08–891.96) | 567.24 ± 171.45 (272.34–827.28) | ||
| Leaf (2 weeks after first flower) | 410.80 ± 42.95 (310.88–495.99) | 359.55 ± 79.40 (227.92–478.53) | ||
| Root (square initiation) | 86.62 ± 27.25 (44.05–127.19) | 91.96 ± 21.52 (58.55–125.74) | ||
| Pollen (flowering) | 11.91 ± 3.03 (6.56–17.48) | 13.18 ± 5.90 (7.85–30.01) | ||
| Fuzzy seed (maturity) | 105.70 ± 12.79 (81.48–127.63) | 117.59 ± 12.24 (98.99–133.69) | ||
| PAT | GHB614 × LLCotton25 × MON 15985 | GHB614 | LLCotton25 | MON 15985 |
| Leaf (4–6 leaf) | 519.38 ± 158.43 (261.28–1029.36) | 461.68 ± 49.44 (401.12–608.19) | ||
| Leaf (square initiation) | 540.34 ± 66.92 (422.13–636.93) | 418.55 ± 43.17 (365.42–502.18) | ||
| Leaf (2 weeks after first flower) | 464.49 ± 43.19 (391.05–543.93) | 416.55 ± 57.51 (355.89–539.01) | ||
| Root (square initiation) | 144.67 ± 43.15 (73.83–194.07) | 153.11 ± 20.87 (115.69–183.99) | ||
| Pollen (flowering) | 1.49 ± 1.06 (0.09–3.81) | 0.74 ± 0.66 (0.08–2.24) | ||
| Fuzzy seed (maturity) | 161.02 ± 19.35 (122.48–193.10) | 152.43 ± 19.53 (104.93–172.19) | ||
| GUS | GHB614 × LLCotton25 × MON 15985 | GHB614 | LLCotton25 | MON 15985 |
| Leaf (4–6 leaf) | 180.76 ± 48.27 (112.71–254.48) | 205.32 ± 19.53 (162.90–232.64) | ||
| Leaf (square initiation) | 247.33 ± 35.71 (201.32–294.17) | 245.48 ± 38.46 (179.05–308.26) | ||
| Leaf (2 weeks after first flower) | 174.58 ± 37.33 (119.31–223.39) | 147.11 ± 41.61 (87.40–210.30) | ||
| Root (square initiation) | 65.23 ± 35.17 (18.24–122.00) | 58.04 ± 13.88 (33.29–78.46) | ||
| Pollen (flowering) | 1.56 ± 0.24 (1.17–2.13) | 1.42 ± 0.24 (< LLOQ–1.98) | ||
| Fuzzy seed (maturity) | 32.06 ± 4.20 (21.77–39.52) | 29.36 ± 3.35 (20.81–34.06) | ||
| NPTII | GHB614 × LLCotton25 × MON 15985 | GHB614 | LLCotton25 | MON 15985 |
| Leaf (4–6 leaf) | 16.09 ± 2.85 (10.86–22.99) | 16.32 ± 1.48 (13.71–18.66) | ||
| Leaf (square initiation) | 15.24 ± 2.53 (11.35–18.99) | 13.79 ± 2.07 (10.65–17.77) | ||
| Leaf (2 weeks after first flower) | 18.55 ± 1.33 (16.29–20.76) | 15.76 ± 1.26 (13.37–17.88) | ||
| Root (square initiation) | 3.88 ± 0.62 (2.42–4.77) | 3.57 ± 0.47 (3.10–4.41) | ||
| Pollen (flowering) | < LOD (NA) | < LOD (NA) | ||
| Fuzzy seed (maturity) | 6.60 ± 0.91 (5.19–8.34) | 6.05 ± 0.62 (4.89–7.52) | ||
Cry: crystal protein; EPSPS: 5‐enolpyruvyl‐shikimate‐3‐phosphate synthase; PAT: phosphinothricin acetyltransferase; GUS: β‐d‐glucuronidase; NPTII: neomycin phosphotransferase II; LOD: limit of detection; LLOQ: lower limit of quantification; NA: not applicable.
Number of samples is n = 15 except for: Cry1Ac in root (n = 6 and n = 10 of GHB614 × LLCotton25 × MON 15985 and MON 15985, respectively); Cry2Ab2 in pollen (n = 12 of GHB614 × LLCotton25 × MON 15985 and MON 15985); GUS in pollen (n = 14 of MON 15985).
Mean.
Standard deviation.
Range.
Reported values for pollen are derived from fresh weight (FW) tissue.