Table D.1.

Genotoxicity Data on (1R,2S,5R)‐isopulegol from EFFA (EFFA, 2016a)

Chemical name [FL‐no:]	Test system in vitro	Test object	Concentrations of substance and test conditions	Result	Reference	Comments
(1R,2S,5R)‐isopulegol [02.067]	Reverse mutation	S. typhimurium TA98, TA100, TA1535 and TA1537	0, 33, 100, 333, 1,000, 2,500 and 5,000 μg/plate	Negativea	Schulz (2010)	Test performed according to OECD Test Guideline 471 and GLP
			0, 10, 33, 100, 333, 1,000 and 2,500 μg/plate	Negativea , b
	Reverse mutation	E. coli WP2uvrA	0, 33, 100, 333, 1,000, 2,500 and 5,000 μg/plate	Negativea
			0, 33, 100, 333, 1,000, 2,500 and 5,000 μg/plate	Negativea , b
	Micronucleus assay	Human peripheral blood lymphocytes	250, 500 and 700 μg/mL	Negativea , c	Roy (2015)	Test performed according to OECD Test Guideline 487 and GLP
			25, 50 and 100 μg/mL	Negatived

FL‐no: FLAVIS number; OECD: Organisation for Economic Co‐operation and Development; GLP: Good Laboratory Practice.

^aWith and without metabolic activation.

^bAssay modified with pre‐incubation.

^c4‐h treatment.

^d24‐h treatment, in the absence of S9‐mix.