Table 4.
Specifications for calcium diglutamate (E 623) according to Commission Regulation (EU) No 231/2012 and JECFA (2006b)
| Commission Regulation (EU) No 231/2012 | JECFA (2006b) | |
|---|---|---|
| Definition | ||
| Assay | Not less than 98.0% and not more than 102.0% on the anhydrous basis | Not less than 98.0% and not more than 102.0% on the anhydrous basis |
| Solubility | Freely soluble in water; practically insoluble in ethanol or ether | Freely soluble in water |
| Description | White, practically odourless crystals or crystalline powder | White, practically odourless crystals or crystalline powder |
| Identification | ||
| Test for glutamic acid (by thin layer chromatography) | Passes test | Passes test |
| Test for calcium | Passes test | Passes test |
| Specific rotation | [α]D 20 between + 27.4° and + 29.2° (for calcium diglutamate with n = 4)(10% solution (anhydrous basis) in 2N HCl, 200 mm tube) | [α]D 20 between + 27.4° and + 29.2° (10% (w/v) solution in 2N hydrochloric acid) |
| Purity | ||
| Water | Not more than 19.0% (for calcium diglutamate with n = 4) (Karl Fischer) | Not more than 19% (Karl Fischer method) |
| Chloride | Not more than 0.2% | Not more than 0.2% |
| Pyrrolidone carboxylic acid | Not more than 0.2% | Passes test |
| Lead | Not more than 1 mg/kg | Not more than 1 mg/kg |