Table A.2.
Estimation of user exposure to endotoxins from the additive l‐tryptophan produced by Escherichia coli DSM 25084, including consideration of using a filter mask FF P2 or FF P3 as a preventative measure
| Calculation | Identifier | Description | Amount | Source |
|---|---|---|---|---|
| a | Endotoxin content IU/g product | 31,600 | Technical dossier | |
| b | Dusting potential (g/m3) | 1.361 | Technical dossier | |
| a × b | c | Endotoxin content in the air (IU/m3) | 43,008 | |
| d | No. of premixture batches made/working day | 40 | EFSA Guidance on user safety (EFSA FEEDAP Panel, 2012b) | |
| e | Time of exposure (s) per production of one batch | 20 | EFSA Guidance on user safety (EFSA FEEDAP Panel, 2012b) | |
| d × e | f | Total duration of daily exposure/worker (s) | 800 | |
| g | Uncertainty factor | 2 | EFSA Guidance on user safety (EFSA FEEDAP Panel, 2012b) | |
| f × g | h | Refined total duration of daily exposure/worker (s) | 1,600 | |
| h/3,600 | i | Refined total duration of daily exposure (h) | 0.44 | |
| j | Inhaled air (m3) per 8‐h working day | 10 | EFSA Guidance on user safety (EFSA FEEDAP Panel, 2012b) | |
| j/8 × i | k | Inhaled air during exposure (m3) | 0.56 | |
| c × k | l | Endotoxin inhaled (IU) during exposure per 8‐h working day | 23,893 | |
| m | Health‐based recommended exposure limit of endotoxin (IU/m3) per 8‐h working day | 90 | Health Council of the Netherlands (2010) | |
| m × j | n | Health‐based recommended exposure limit of total endotoxin exposure (IU) per 8‐h working day | 900 | |
| l/10 | Endotoxins inhaled (IU) per 8‐h working day reduced by filter mask FF P2 (reduction factor 10) | 2,389 | ||
| l/20 | Endotoxins inhaled (IU) per 8‐h working day reduced by filter mask FF P3 (reduction factor 20) | 1,195 |