Table E.2.

Subacute and subchronic toxicity studies

Chemical name [FL‐no]	Species/sex No/group	Route	Dose levels (mg/kg per day)	Duration	NOAEL (mg/kg per day)	Reference	Comments
(Acetone [07.050])	Rat/M,F 10	Drinking water	0, 250, 500, 1,000, 2,000, 5,000	13 weeks	1,000a	Diet (1991)	c NTP study
	Mouse/M,F 10	Drinking water	0, 312, 625, 1,250, 2,500, 5,000 (M) 0, 625, 1,250, 2,500, 5,000, 12,500 (F)	13 weeks	2,500a	Dietz (1991)	c NTP study
	Rat/M,F 30	Gavage	0, 100, 500, 2,500	90 days	100	Sonawane et al. (1986)	c Meeting abstract
	Rat/NR 3	Drinking water	1,000	4 weeks	1,000a , b	Spencer et al. (1978)	c Examinations were limited to specific neurotoxic effects. No other parameter was monitored
(Isopropyl alcohol [02.079])	Human/M 8	Oral	0, 2.6, 6.4	6 weeks	6.4b	Wills et al. (1969)	c Paper published in a peer‐reviewed journal
	Rat/M 22	Drinking water	0, 870, 1,280, 1,680, 2,520	12 weeks	870	Pilegaard and Ladefoged (1993)	c Good quality study
Pentan‐3‐one [07.084]	Rat/F 5	Drinking water	0, 1,860	120 days	Not detected (< 1,860)	Union Carbide Corp. (1977)	Good quality unpublished report. Focused on neurotoxic effect
(2‐Heptanone [07.002])	Rat/M,F 15	Gavage (dissolved in corn oil)	0, 20, 100, 500	13 weeks	20	Gaunt et al. (1972)	c Good quality study – peer‐reviewed journal
	Rat/NR 5	Drinking Water	0, 500	12 weeks	500a , b	Spencer et al. (1978)	c Good quality study – peer‐reviewed journal
(3‐Heptanone [07.003])	Rat/M 2	Gavage	0, 250, 500, 1,000, 2,000, 4,000	14 weeks	1,000	O'Donoghue et al. (1984)	c Good quality study – peer‐reviewed journal
	Rat/F NR	Drinking Water	1,000	120 days	1,000a	Homan and Maronpot (1978)	c Meeting abstract
	Rat/F 5	Drinking water	0, 27	120 days	27b	Union Carbide Corp. (1977)	Good quality unpublished report. Focused on neurotoxic effect
(4‐Heptanone [07.058])	Rat/M 8	Gavage	0, 1,000	90 days	Not detected (< 1,000)	O'Donoghue and Krasavage (1980)	c Good quality unpublished report
	Rat/M 3	Gavage (undiluted)	0, 1,000, 2,000, 4,000	3 weeks	Not detected (< 1,000)	Krasavage and O'Donoghue (1979)	c Good quality unpublished report
(2‐Nonanone [07.020])	Rat/M 3	Gavage (undiluted)	0, 1,000, 2,000, 4,000	3 weeks	Not detected (< 1,000)	Krasavage and O'Donoghue (1979)	c Good quality unpublished report
	Rat/M 8	Gavage	0, 2,000	90 days	Not detected (< 2,000)	O'Donoghue and Krasavage (1980)	c Good quality unpublished report
(4‐Methyl‐2‐pentanone [07.017])	Rat/M,F 5	Drinking water	0, 1,040	120 days	Not detected (< 1,040)	Union Carbide Corp. (1977)	Good quality unpublished report. Focused on neurotoxic effect
	Rat/F NR	Drinking water	1,000	120 days	1,000b	Homan and Maronpot (1978)	c Meeting abstract
Methyl‐5‐heptan‐3‐one [07.182]	Rat/M 5	Gavage (in distilled water)	82, 410, 820	13 weeks (5 days/week)	82	IBM Corp. (1989)	Good quality unpublished report submitted to EPA
(2,6‐Dimethyl‐4‐heptanone [07.122])	Rat/M 8	Gavage	0, 2,000	90 days	Not detected (< 2,000)	O'Donoghue and Krasavage (1980)	c Good quality unpublished report
(5‐Methyl‐5‐hexen‐2‐one [07.100])	Rat/M,F 5	Diet	0, 10	14 days	10b	Gill and Van Miller (1987)	d GLP study – unpublished report
(2,6,10‐Trimethyl‐2,6,10‐pentadecatrien‐14‐one [07.114])	Rat/M,F 5	Oral (gavage in maize oil)	0, 0.35, 3.5	14 days	3.5	deGroot et al. (1974)	d TNO Unpublished Report
9‐Decen‐2‐one [07.262]	Rat/M,F 5	Oral (gavage in corn oil)	0, 250, 500, 1,000	28 days	1,000e	Flavour Industry (2009)	Good study, OECD 407

FL‐no: FLAVIS number; NOAEL: no observed adverse effect level.

NR = sex not reported; M = Male; F = Female.

^aConcentrations converted to mg/kg bw per day using conversion table for test chemical treatment doses used in PAFA (FDA, 1993).

^bThis study was performed at a single dose level that produced no adverse effects.

^cSummarised by JECFA, 51st meeting (JECFA, 1999a).

^dSummarised by JECFA 59th meeting (JECFA, 2003).

^eThe highest dose tested.