| HPLC‐FD | LC–MS | LC–MS/MS | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Grains for human consumption | Grain milling products | Grains for human consumption | Grain milling products | Grains and grain‐based products | Grains for human consumption | Grain milling products | |||||||||||||||
| Limits of quantification (μg/kg) | Limits of quantification (μg/kg) | Limits of quantification (μg/kg) | |||||||||||||||||||
| N | Min | Max | N | Min | Max | N | Min | Max | N | Min | Max | N | Min | Max | N | Min | Max | N | Min | Max | |
| Ergometrine | 18 | 6.2 | 6.3 | 12 | 6.2 | 6.3 | 8 | 1.0 | 1.0 | 9 | 1.0 | 1.0 | 2 | 2.0 | 20.0 | 410 | 0.1 | 20.0 | 665 | 0.1 | 20.0 |
| Ergosine | 18 | 12.5 | 12.5 | 12 | 12.5 | 12.5 | 8 | 1.0 | 1.0 | 9 | 1.0 | 1.0 | 2 | 2.0 | 20.0 | 412 | 0.1 | 20.0 | 666 | 0.1 | 20.0 |
| Ergocornine | 18 | 12.5 | 12.5 | 12 | 12.5 | 12.5 | 8 | 1.0 | 1.0 | 9 | 1.0 | 1.0 | 2 | 2.0 | 20.0 | 410 | 0.1 | 20.0 | 662 | 0.1 | 20.0 |
| Ergotamine | 18 | 12.5 | 12.5 | 12 | 12.5 | 12.5 | 8 | 1.0 | 1.0 | 9 | 1.0 | 1.0 | 2 | 4.0 | 20.0 | 411 | 0.1 | 20.0 | 666 | 0.1 | 20.0 |
| alpha‐Ergocryptine | 18 | 12.5 | 12.5 | 12 | 12.5 | 12.5 | – | – | – | – | – | – | – | – | – | 104 | 0.1 | 10.0 | 94 | 0.1 | 10.0 |
| beta‐Ergocryptine | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 5 | 0.1 | 0.1 | 11 | 0.1 | 0.1 |
| Ergocristine | 18 | 12.5 | 12.5 | 12 | 12.5 | 12.5 | 8 | 1.0 | 1.0 | 9 | 1.0 | 1.0 | 2 | 2.0 | 20.0 | 411 | 0.1 | 20.0 | 665 | 0.1 | 20.0 |
| Ergometrinine | 14 | 12.2 | 12.5 | 11 | 6.2 | 12.5 | 8 | 1.0 | 1.0 | 9 | 1.0 | 1.0 | 2 | 1.0 | 20.0 | 365 | 0.1 | 20.0 | 649 | 0.1 | 20.0 |
| Ergosinine | 14 | 12.2 | 12.5 | 11 | 12.5 | 12.5 | 8 | 1.0 | 1.0 | 9 | 1.0 | 1.0 | 2 | 4.0 | 20.0 | 366 | 0.1 | 20.0 | 662 | 0.1 | 20.0 |
| Ergocorninine | 18 | 12.5 | 12.5 | 12 | 12.5 | 12.5 | 8 | 1.0 | 1.0 | 9 | 1.0 | 1.0 | 2 | 4.0 | 20.0 | 410 | 0.1 | 20.0 | 662 | 0.1 | 20.0 |
| Ergotaminine | 18 | 12.5 | 12.5 | 12 | 12.5 | 12.5 | 8 | 1.0 | 1.0 | 9 | 1.0 | 1.0 | 2 | 4.0 | 20.0 | 410 | 0.1 | 20.0 | 662 | 0.1 | 20.0 |
| alpha‐Ergocryptinine | 18 | 12.5 | 12.5 | 12 | 12.5 | 12.5 | – | – | – | – | – | – | – | – | – | 87 | 0.1 | 3.0 | 66 | 0.1 | 2.0 |
| Ergocristinine | 18 | 12.5 | 12.5 | 12 | 12.5 | 12.5 | 8 | 1.0 | 1.0 | 9 | 1.0 | 1.0 | 2 | 4.0 | 20.0 | 410 | 0.1 | 20.0 | 662 | 0.1 | 20.0 |
| Ergocryptine (sum of epimers) | – | – | – | – | – | – | 8 | 1.0 | 1.0 | 9 | 1.0 | 1.0 | 2 | 2.0 | 20.0 | 306 | 1.0 | 20.0 | 568 | 1.0 | 20.0 |
| Ergocryptinine (sum of epimers) | – | – | – | – | – | – | 8 | 1.0 | 1.0 | 9 | 1.0 | 1.0 | 2 | 4.0 | 20.0 | 323 | 1.0 | 20.0 | 596 | 1.0 | 20.0 |
HPLC‐FD: high‐performance liquid chromatography with fluorescence detection; LC–MS: liquid chromatography–mass spectrometry; LC–MS/MS: liquid chromatography–tandem mass spectrometry.