Agents	Effects	Subjects	Conditions
Additive/single active substances: trans‐anethole and thymol	Short‐term toxicity (mortality, clinical effects, performance parameters, haematology, blood chemistry, histopathology when needed)	Target species	Dietary exposure at recommended dose in feed (150 mg/kg feed) – Establishment of a safe dose via tolerance studies (x10 overdosing)
Single active substance: trans‐anethole	Effects on liver enzymes and histopathology. Liver effects for trans‐anethole in laboratory animals	Target species, Consumers	Dietary exposure via residues (data available from tolerance study, x10)
Single active substances: trans‐anethole and thymol	Irritation, skin sensitisation	Users/workers	Exposure by inhalation, contact, systemic exposure
Single active substances: trans‐anethole and thymol	Short‐term effects (LC50, EC50) and long‐term effects (NOEC)	Terrestrial and aquatic organisms in the environment	Exposure via manure containing residues or non consumed feed
Single active substance: estragole	Genotoxicity	All subjects	See above
Additive/single active substances: trans‐anethole and thymol	Improving animal performances	Target species	Dietary exposure at the proposed use level
	Digestibility enhancer