Table 3.
Genetic elements in the expression cassettes of the events stacked in maize MON 87427 × MON 89034 × NK603
| Event | Promoter | 5′ UTR | Transit peptide | Coding region | Terminator |
|---|---|---|---|---|---|
| MON 87427 |
35S (CaMV) |
– |
CTP2 (Arabidopsis thaliana) |
CP4 epsps (Agrobacterium sp.) |
nos (Agrobacterium tumefaciens) |
| MON 89034 |
35S (CaMV) |
CAB (Triticum sp.) |
– |
cry1A.105 (B. thuringiensis) |
Hsp17 (Triticum sp) |
|
35S (FMV) |
– |
CTP (Zea mays) |
cry2Ab2 (B. thuringiensis) |
nos (A. tumefaciens) |
|
| NK603 |
act1 (Oryza sativa) |
– |
CTP2 (A. thaliana) |
CP4 epsps (Agrobacterium sp.) |
nos (A. tumefaciens) |
|
35S (CaMV) |
– |
CTP2 (A. thaliana) |
CP4 epsps L214P (Agrobacterium sp.) |
nos (A. tumefaciens) |
CaMV: cauliflower mosaic virus; UTR: untranslated region; CTP: chloroplast transit peptide.
(–): When no element was specifically introduced to optimise expression.