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. Author manuscript; available in PMC: 2020 Feb 10.
Published in final edited form as: Cell Rep. 2019 Dec 17;29(12):3997–4009.e5. doi: 10.1016/j.celrep.2019.11.070

Figure 4. NS1 Evolutionary Directions within the Viral Library Show Species-Dependent Profiles that Preferentially Overrepresent Specific NS1 Clades.

Figure 4.

Amino acid differences across all NS1 sequences were integrated in a percentage identity matrix, and relative distances were plotted using x and y coordinates. Averages of triplicates expressed as the log2 fold induction over the initial relative proportion of barcode reads found in the initial viral mix (input) were plotted on the z axis. (A–F) Individual plots in mice (A and B), MDCK cells (C and D), and allantoic fluid (E and F) were generated. Viruses containing allele A (A, C, E) or allele B (B, D, F) NS1 were plotted separately in each case for visualization purposes.