KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-CRH | Peninsula | Cat# T4037; RRID:AB_518252 |
| chicken anti-β-gal | Abcam | Cat# Ab9361; RRID:AB_307210 |
| rabbit anti GFAP | Phosphosolution | Cat# 620-GFAP; RRID:AB_2492124 |
| goat anti-rabbit IgG | Thermo Fisher Scientific | Cat# A-11012; RRID:AB_2534079 |
| goat anti-rabbit IgG | Thermo Fisher Scientific | Cat# SA5-10033; RRID:AB_2556613 |
| goat anti-chicken IgY | Thermo Fisher Scientific | Cat# A32933; RRID:AB_2762845 |
| Bacterial and Virus Strains | ||
| AAV9-EF1a-DIO-hChR2(H134R)-mCherry | Vector Biolabs | Cat. No: VB4651 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Rhod-2 AM | Life Technologies | Cat# R1244 |
| GDP-β-s | Sigma-Aldrich | Cat# G7637 |
| 6,7-dinitroquinoxaline-2,3-dione | Tocris | Cat# 0189 |
| DL-2-amino-5-phosphonopentanoic acid | Tocris | Cat# 0105 |
| Picrotoxin | Sigma-Aldrich | P1675; CAS: 124-87-8 |
| Experimental Models: Organisms/Strains | ||
| Tg(CRH-EGFP)HS57Gsat/Mm | MMRRC | RRID:MMRRC_017058-UCD |
| tetO-dnSNARE mouse | Haydon Lab | N/A |
| GFAP-tTA mouse | Haydon Lab | N/A |
| B6.Cg-7630403G23RikTg(Th-cre)1Tmd/J | Jackson Lab | RRID:IMSR_JAX:008601 |
| TH-cre/CRH-eGFP mouse | In house | N/A |
| Software and Algorithms | ||
| Prism v. 8 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| Mini Analysis | Synaptosoft, Inc. | http://www.synaptosoft.com/MiniAnalysis/ |