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. 2020 Feb 10;3(2):e201900635. doi: 10.26508/lsa.201900635

Figure S8. Effects of glucose concentrations on MEFs.

Figure S8.

(A) Effects of low glucose was evaluated between 0 and 5 mM in the presence of 10% dialyzed FBS with or without 1 mM pyruvate supplement. Although both control and Crk;Crkl-deficient MEFs died without glucose within 24 h, more control MEFs survived compared with Crk;Crkl-deficient MEFs. The presence of pyruvate did not rescue MEFs deprived of glucose in control or Crk;Crkl-deficient MEFs (CTRL and 4OHT, respectively). (B) Effects of low glucose was evaluated between 0 and 1 mM. Note that although both control and Crk;Crkl-deficient MEFs tolerated low glucose down to 0.1 mM for a period of 24 h, the 0.1 mM concentration appeared to restrict cell proliferation particularly in control MEFs. From these results, we decided to use 0.1 mM as a glucose starvation concentration.