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. Author manuscript; available in PMC: 2020 Feb 10.
Published in final edited form as: Cell Rep. 2020 Jan 14;30(2):421–431.e7. doi: 10.1016/j.celrep.2019.12.044

Figure 2. Observation of Ω-Shaped Membrane Profile Shrinking and Enlargement while Releasing FFN511.

Figure 2.

(A) Setup diagram. Vesicles were loaded with FFN511 (red, pseudo-color), the PM was labeled with PHG (green), and ICa and Cm were recorded via a pipette.

(B–E) FPH, FFN511 fluorescence (FFFN; normalized to baseline), PH-Ω height (H; circles) and width (W; triangles), and sampled images at times indicated with lines showing release of FFN511 for shrink fusion (B), partial shrink fusion (C), same-size fusion (D), and enlarge fusion (E).

(F) 20%–80% decay time of FFN511 (TFFN_decay, mean + s.e.m.) for shrink fusion (8 events), partial shrink fusion (13 events), same-size fusion (53 events), and enlarge-fusion (18 events). Data are from 71 cells under STED xz/yfix imaging of PHG/FFN511. ***p < 0.001; ANOVA test.