. 2020 Feb 5;76(Pt 2):94–102. doi: 10.1107/S2053230X20001132

Table 1. Crystallization conditions for EcFtsZ(L178E)–nucleotide structures.

	EcFtZ(L178E)–GDP	EcFtsZ(L178E)–GTP
Method	Hanging-drop vapor diffusion	Hanging-drop vapor diffusion
Plate type	24-well plates	24-well plates
Temperature (K)	298	298
Protein concentration (mg ml⁻¹)	37	30
Buffer composition of protein solution	50 mM Tris–HCl pH 7.9, 50 mM KCl, 10% glycerol, 1 mM EDTA	50 mM Tris–HCl pH 7.9, 50 mM KCl, 10% glycerol, 1 mM EDTA
Composition of reservoir solution	50 mM sodium cacodylate pH 6.5, 11% PEG 8000, 150 mM calcium acetate	50 mM sodium cacodylate pH 6.5, 13% PEG 8000, 150 mM calcium acetate
Volume and ratio of drop	5 µl:5 µl	5 µl:5 µl
Volume of reservoir (ml)	0.75	0.75