Fig 2. Taf4a vs Taf4b enrichment in the germ cells of the mouse embryonic gonad.

Gene mRNA expression levels of Taf4b (A-B) and Taf4a (C-D) in female and male embryonic germ cells (“G”) and somatic cells (“S”) from E9.5 to E18.5 indicate that Taf4b is significantly (* = log2FC > |0.25|, p-adj. < 0.05) and consistently enriched in germ cells from E11.5 to E18.5 in comparison to somatic cells. Error bars indicate ± standard error of the mean (SEM). (E) Western blot protein signal of TAF4a and TAF4b in cells sorted from E13.5 Oct4-EGFP gonads. Mouse Vasa Homology (MVH) is a germ cell marker, which is only detected in the GFP⁺ lanes of the female and male samples, indicating that the GFP^- lane contains only somatic cells. TAF4a protein signal is detected in all lanes (both germ and somatic cell types), whereas TAF4b is only detected in the GFP⁺ lane (germ cells). β-Actin is a protein loading control. Two female mice and two male mice were used to obtain roughly 35,000 pooled cells for each lane. (F) Western blot experiment performed in 3 replicates was quantified for relative protein signal. TAF4b protein levels between GFP⁺ and GFP^- cells in female E13.5 ovary are significantly (* = p < 0.05, unpaired t-test) different. MVH is significantly different for both females and males. Error bars indicate ± SEM.