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. 2020 Jan 23;9:e52572. doi: 10.7554/eLife.52572

Figure 2. Glutamatergic and catecholaminergic innervations within the pFRG region.

(A, B) Single case showing the close apposition of glutamatergic (VGlut2; green) and/or catecholaminergic (TH; red) terminals in the pFRG neurons (NeuN; blue). (C) Rostro-caudal distribution of glutamatergic (VGlut2; green) and catecholaminergic (TH; red) terminal in the pFRG region (Bregma level: −11.60 at 10.64 mm). Note the overlap between the VGlut2 and TH terminals. (D) VGlut2-immunoreactivity density in a rostro-caudal distribution (Bregma level from −11.6 to −10.64 mm). (E) TH-immunoreactivity density in a rostro-caudal distribution (Bregma level from −11.6 to −10.64 mm). Abbreviations: NeuN, nuclear neuronal marker; Sp5, spinal trigeminal tract; TH, tyrosine hydroxylase; VGlut2, vesicular glutamate transporter 2; VII, facial motor nucleus;. Scale bar in panel (A) = 1 mm, in panel (B) = 10 μm, and in panel (C) = 100 μm.

Figure 2.

Figure 2—figure supplement 1. Total number of NeuN neurons located in the pFRG region.

Figure 2—figure supplement 1.

(A) Photomicrography showing immunoreactive markers for tyrosine hydroxylase (TH, red) NeuN (blue) and VGlut2 (green) expression in the pFRG region and a split figure showing only NeuN expression in pFRG. (B) Rostro-caudal distribution of NeuN immunoreactive cells located in the pFRG region. Abbreviations: pFRG, parafacial respiratory group; Sp5, spinal trigeminal tract; VII, facial motor nucleus. Scale bar = 100 μm.