Figure 1. Circulating EVs are diagnostic factor in ME/CFS patients correlating to CRP and BAP.
A-D) Characterization of circulating EVs from ME/CFS patients, first group (ME/CFS 1) and second group (ME/CFS 2), or healthy controls (HC) via flow cytometry. A) Histograph of unstained or stained circulating EVs from ME/CFS patient. B) Quantification of calcein-positive circulating EVs. C) Dynamic light scattering analysis of isolated EVs from ME/CFS patients (ME/CFS) or HC. D) Transmission electron microscopy of isolated EVs from ME/CFS patients. E) Dot plot of circulating EVs and CRP level. F) Dot plot of circulating EVs and BAP level. G) ROC curve with circulating EVs, CRP level, BAP level, and d-Roms level. HC: healthy individual. Values represent mean ± SEM. ***P < 0.001.