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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Brain Behav Immun. 2019 Nov 22;84:45–58. doi: 10.1016/j.bbi.2019.11.007

Figure 6. Methadone Increases TLR4 and Myd88 Inflammatory Gene Expression in the Developing Brain.

Figure 6.

Osmotic minipumps containing methadone or saline were implanted in pregnant rats on E16. Brain cortex on postnatal day 10 (P10) was assayed for levels of TLR4 (A) and Myd88 (B) gene expression. Methadone significantly increased cerebral TLR4 and Myd88 mRNA expression compared to cortex from pups born to dams with osmotic minipumps containing saline. These data are consistent with a cerebral inflammatory microenvironment during a crucial period of neurodevelopment. *p<0.05, **p<0.01, n=4–6/group.