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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Brain Behav Immun. 2019 Nov 22;84:45–58. doi: 10.1016/j.bbi.2019.11.007

Figure 7. Perinatal Methadone Exposure Causes Sustained Neuroinflammation.

Figure 7.

Osmotic minipumps containing methadone or saline were implanted in pregnant rats on E16. Cerebral cortex collected on postnatal day 10 (P10) was assayed using a translational multiplex electrochemiluminescent platform to quantify cytokine and chemokine levels. Those pups exposed to methadone had significantly increased IL-1β (A), and CXCL1 (D) compared to saline control pups. Levels of TNFα (B) and IL-6 remained unchanged. These data reflect a robust and prolonged increase in pro-inflammatory proteins the brain during a critical period of neurodevelopment. *p<0.05, **p<0.01, ***p<0.001, n=6–7/group.