TABLE 4.

The location of the most significant QTLs, limited to the top 5, which were associated with back length, and the genes located within these QTLs within each breed.

				No of suggestive and significant SNPs	Most significant SNP		Allele frequency of positive allele
Breed	Chr	Start	End			P-Value	AA	CH	HE	LM	SI	Candidate genes within this QTL
Angus	6	37939769	40455422	70	38443019a	5.79 × 10–7	0.139	0.000	0.847	0.207	0.311	PKD2, SPP1, MEPE, LAP3, NCAPG*, LCORL*
	6	40762050	42494936	24	41262050b	8.44 × 10–7	0.032	0.003	0.000	0.000	0.000	SLIT2*, PACRGL, KCNIP4*
	9	11789073	12803143	4	12298383a	1.17 × 10–6	0.008	0.032	0.979	0.987	0.969	RIMS1, KCNQ5
	12	84208854	85283107	29	84720853a	6.13 × 10–8	0.949	0.000	0.013	0.035	0.981
	13	68993173	70000878	3	69495192a	6.75 × 10–7	0.026	0.000	0.032	0.073	0.060
Charolais	2	1	10036842	5525	6808074a	3.96 × 10–48	0.000	0.079	0.000	0.972	0.996	WDR75, ASNSD1∧, ARHGEF4∧, MYO7B∧, NAB1∧, MFSD6∧, MSTN∧, PMS1∧, ORMDL1∧, COL3A1∧, COL5A2∧, ANKAR∧, SLC40A1∧
	14	33353270	34356964	4	33853270a	1.19 × 10–7	0.000	0.013	0.000	0.000	0.992	ARFGEF1, CPA6, PREX2
	14	44425358	45430890	3	44928243a	7.51 × 10–7	0.209	0.273	0.605	0.423	0.423	STMN2, HEY1, MRPS28
	28	19217733	21371343	36	19836248a	1.01 × 10–7	0.418	0.784	0.575	0.583	0.626	NRBF2, REEP3*
	28	30350477	31864396	38	31332353a	6.88 × 10–9	0.450	0.859	0.629	0.426	0.629	KAT6B*, DUPD1, DUSP13, VDAC2
Hereford	4	1	910718	5	223774a	1.15 × 10–6	0.975	0.984	0.981	0.981	0.000	VSTM2A*
	4	37522586	38567213	13	38055263a	2.31 × 10–6	0.959	0.283	0.130	0.851	0.201	PCLO*
	8	85462715	87578203	16	86646431a	1.63 × 10–6	0.000	0.000	0.998	0.000	0.000	OGN, ASPN, ECM2, IPPK, BICD2, FGD3, NINJ1, BARX1*,PTPDC1*
	14	30747311	31758061	7	31247311a	3.41 × 10–6	0.429	0.333	0.485	0.636	0.648	BHLHE22, MTFR1
	18	29621954	30630622	5	30130622a	8.58 × 10–7	0.996	0.995	0.996	0.985	0.010	CDH8
Limousin	1	66063243	67175049	15	66587440b	2.16 × 10–7	0.002	0.030	0.983	0.997	0.018	GTF2E1, STXBP5L, POLQ*, FBXO40, HCLS1, GOLGB1
	3	24752329	26688150	3	26188150d	9.48 × 10–7	0.000	0.897	0.908	0.917	0.888	SPAG17*, WDR3, MAN1A2, VTCN1*, TRIM45, TTF2, CD101, PTGFRN
	6	32025422	34384319	1058	33661101a	5.14 × 10–13	0.753	0.904	0.407	0.142	0.259	ATOH1
	6	36996616	41253691	469	38792702b	4.24 × 10–13	0.097	0.000	0.105	0.091	0.000	ABCG2∧, PKD2∧, SPP1*, MEPE, LAP3, NCAPG∧, LCORL∧, SLIT2
	21	33476048	34502357	6	33999605a	1.55 × 10–6	0.006	0.017	0.005	0.017	0.005	CSPG4, SNX33, IMP3, PTPN9
Simmental	15	77047714	78087312	9	77558153b	5.09 × 10–7	0.811	0.000	0.270	0.000	0.264	DGKZ, ATG13, ARHGAP1, ZNF408, CKAP5*
	16	10050545	11308116	5	10550545a	6.88 × 10–7	0.000	0.000	0.000	0.000	0.981
	17	62751558	63784022	12	63254862b	1.24 × 10–6	0.047	0.940	0.977	0.930	0.969	LHX5*, PLDB2, OAS2, OAS1Y, OAS1X
	20	43798108	44854685	5	44298108a	2.56 × 10–6	0.042	0.069	0.240	0.074	0.109
	21	10803227	11841095	7	11303227a	2.88 × 10–6	0.998	0.012	0.980	0.006	0.994	NR2F2

AA, Angus; CH, Charolais; HE, Hereford; LM, Limousin; SE, Simmental; HF, Holstein-Friesian. Superscript denotes SNP classification:^aintergenic, ^bintron, ^cupstream gene variant, ^ddownstream gene variant. Symbols denote the significance of SNPs within genes: * gene contained at least one suggestive (p ≤ 1 × 10^–5) SNP ^∧ gene contained at least one significant (p ≤ 1 × 10^–8) SNP.